Reconstruction and Analysis of the Microtubule Spindle Using Electron Tomography
Microtubules are tube-like polymers that play an important role in cell division. They form the spindle, a microtubule assembly that segregates the chromosomes. One important method for investigating microtubules in cells is electron tomography. Thick (300nm) serial sections of cells are prepared for electron microscopy and imaged on a CCD camera. With the use of image processing techniques, we are able to automatically extract the centerlines of microtubules within a section. In this project we will develop methods and software for a quantitative analysis of full mitotic spindles. These methods will allow joining electron tomograms and extracted microtubule centerlines within (in xy) and across (z) physical sections to create a geometric model of the entire spindle, which is then visually and quantitatively analyzed.
Publikationen
2023 |
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Robert Kiewisz, Daniel Baum, Thomas Müller-Reichert, Gunar Fabig | Serial-section electron tomography and quantitative analysis of the microtubule organization in 3D-reconstructed mitotic spindles | Bio-protocol, 13(20), 2023 |
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2022 |
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Alejandra Laguillo-Diego, Robert Kiewisz, Carlos Martí-Gómez, Daniel Baum, Thomas Müller-Reichert, Isabelle Vernos | MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles | Molecular Biology of the Cell, 34(1), 2022 |
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Robert Kiewisz, Gunar Fabig, William Conway, Daniel Baum, Daniel Needleman, Thomas Müller-Reichert | Three-dimensional structure of kinetochore-fibers in human mitotic spindles | eLife, Vol.11, p. e75459, 2022 |
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2021 |
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Ina Lantzsch, Che-Hang Yu, Yu-Zen Chen, Vitaly Zimyanin, Hossein Yazdkhasti, Norbert Lindow, Erik Szentgyoergyi, Ariel M Pani, Steffen Prohaska, Martin Srayko, Sebastian Fürthauer, Stefanie Redemann | Microtubule reorganization during female meiosis in C. elegans | eLife, Vol.10, p. e58903, 2021 |
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Norbert Lindow, Florian Brünig, Vincent J. Dercksen, Gunar Fabig, Robert Kiewisz, Stefanie Redemann, Thomas Müller-Reichert, Steffen Prohaska, Daniel Baum | Semi-automatic stitching of filamentous structures in image stacks from serial-section electron tomography | Journal of Microscopy, 284(1), pp. 25-44, 2021 |
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2020 |
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Norbert Lindow, Florian Brünig, Vincent J. Dercksen, Gunar Fabig, Robert Kiewisz, Stefanie Redemann, Thomas Müller-Reichert, Steffen Prohaska, Daniel Baum | Semi-automatic stitching of filamentous structures in image stacks from serial-section electron tomography | bioRxiv, 2020 |
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Gunar Fabig, Robert Kiewisz, Norbert Lindow, James A. Powers, Vanessa Cota, Luis J. Quintanilla, Jan Brugués, Steffen Prohaska, Diana S. Chu, Thomas Müller-Reichert | Sperm-specific meiotic chromosome segregation in C. elegans | eLife, Vol.9, p. e50988, 2020 |
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2019 |
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Norbert Lindow, Florian Brünig, Vincent J. Dercksen, Gunar Fabig, Robert Kiewisz, Stefanie Redemann, Thomas Müller-Reichert, Steffen Prohaska | Semi-automatic Stitching of Serial Section Image Stacks with Filamentous Structures | ZIB-Report 19-30 |
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2018 |
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Stefanie Redemann, Ina Lantzsch, Norbert Lindow, Steffen Prohaska, Martin Srayko, Thomas Müller-Reichert | A switch in microtubule orientation during C. elegans meiosis | Current Biology, 2018 (preprint available as ZIB-Report 18-34) |
PDF (ZIB-Report)
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Norbert Lindow, Stefanie Redemann, Florian Brünig, Gunar Fabig, Thomas Müller-Reichert, Steffen Prohaska | Quantification of three-dimensional spindle architecture | Methods in Cell Biology Part B, Academic Press, pp. 45-64, 2018 (preprint available as ZIB-Report 18-07) |
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Felix Herter | Supervised Classification of Microtubule Ends: An Evaluation of Machine Learning Approaches | Master's thesis, Freie Universität Berlin, Daniel Baum, Norbert Lindow (Advisors), 2018 |
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2017 |
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Stefanie Redemann, Johannes Baumgart, Norbert Lindow, Michael Shelley, Ehssan Nazockdast, Andrea Kratz, Steffen Prohaska, Jan Brugués, Sebastian Fürthauer, Thomas Müller-Reichert | C. elegans chromosomes connect to centrosomes by anchoring into the spindle network | Nature Communications, 8(15288), 2017 |
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2015 |
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Britta Weber | Reconstruction of Microtubule Centerlines from Electron Tomograms | Doctoral thesis, Freie Universität Berlin, Knut Reinert (Advisor), 2015 |
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2014 |
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Britta Weber, Erin M. Tranfield, Johanna L. Höög, Daniel Baum, Claude Antony, Tony Hyman, Jean-Marc Verbavatz, Steffen Prohaska | Automated stitching of microtubule centerlines across serial electron tomograms | PLoS ONE, p. e113222, 2014 (preprint available as ZIB-Report 14-41) |
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Stefanie Redemann, Britta Weber, Marit Möller, Jean-Marc Verbavatz, Anthony Hyman, Daniel Baum, Steffen Prohaska, Thomas Müller-Reichert | The Segmentation of Microtubules in Electron Tomograms Using Amira | Mitosis: Methods and Protocols, pp. 261-278, Springer, 2014 |
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2012 |
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Britta Weber, Garrett Greenan, Steffen Prohaska, Daniel Baum, Hans-Christian Hege, Thomas Müller-Reichert, Anthony Hyman, Jean-Marc Verbavatz | Automated tracing of microtubules in electron tomograms of plastic embedded samples of Caenorhabditis elegans embryos | Journal of Structural Biology, 178(2), pp. 129-138, 2012 |
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2011 |
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Britta Weber, Marit Möller, Jean-Marc Verbavatz, Daniel Baum, Hans-Christian Hege, Steffen Prohaska | Fast Tracing of Microtubule Centerlines in Electron Tomograms | BioVis 2011 Abstracts, 1st IEEE Symposium on Biological Data Visualization, 2011 |
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